Preliminary analysis on genetic variation of Iphigenia indica by RAPD
- VernacularTitle:丽江山慈菇RAPD遗传变异初探
- Author:
Haiying WANG
;
Hong YU
- Publication Type:Journal Article
- Keywords:
Iphigenia indicaKunth;
RAPD;
genetic variation
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To study on the genetic diversity and genetic structure of Iphigenia indica Kunth. Methods Random amplified polymorphic DNA (RAPD) was applied to detect DNA fingerprints of three populations of I. indicafrom Yunnan Province. Results Twenty primers were screened, and a total of 120 DNA fragments were amplified ranging from 0.2-3 kb, among which 71 (59.17%) were polymorphic. The average number of DNA band produced by each primer was 3.55. The Shannon index was (0.199 4) in Ca population, 0.200 7 in Cb population, 0.254 8 in Cc population, respectively; the average value of populations was 0.218 3. The Shannon index was 0.288 6 in species. Nei's genetic identity was 0.930 9 between Ca and Cb, 0.932 7 between Ca and Cc, 0.946 6 between Cb and Cc. G_(st) within population was (0.204 4.) Conclusion For the establishment of protective tactic and measure, and of the standard of good agriculture practice (GAP) growth and heredity breeding, RAPD analysis provides I. indicawith theoretical foundation and basal data.
