EFFECT OF VELVET ANTLER POLYPEPTIDE(VAP) ON DIFFERENTIATION   OF RAT BRAIN-DERIVED STEM CELLS IN VITRO

VernacularTitle:鹿茸多肽对胎大鼠脑神经干细胞体外诱导分化的实验研究
Author: Dong CHEN ; Xiaoting MENG ; Jiamei LIU ; Lei CHEN ; Laijin LU
Publication Type:Journal Article
Keywords: Neural stem cells; Velvet antler polypeptide(VAP); Differentiation; Neuron; Fetal rat
From: Acta Anatomica Sinica 1955;0(03):-
CountryChina
Language:Chinese
Abstract: Objective To investigate the probabilities of brain-derived stem cells from fetal rats differentiating into neurons and astrocytes by velvet antler polypeptide(VAP) in vitro. Methods Neural stem cells from E12-14d rats were cultured for 7 days until neural stem cells (NSCs) aggregations were formed into neurospheres. The neurospheres were cultured at different concentrations of VAP, and immunocytochemistry was used to detect the differentiation of neural stem cells. Results The differentiated cells in 50?g/L VAP group are more than that in control group; the number of NSE positive cells in 50?g/L,100?g/L and 200?g/L groups is more than that in control group.Conclusion Neural stem cells can be successfully induced into neurons by VAP in vitro, which could provide a basis for regeneration of nerve system.;