INVESTIGATION OF RELATIONSHIP BETWEEN EXPRESSION OF MANNOSE-LIGAND RECEPTOR ON CAPACITATED HUMAN SPERM IN VITRO AND ACROSOME REACTION INDUCED BY SOLUBILIZED MOUSE ZONA PELLUCIDA
- VernacularTitle:人精子甘露糖受体表达与顶体反应的关系
- Author:
Yunxiu LIN
;
Yiping JIANG
- Publication Type:Journal Article
- Keywords:
Mannose-ligand receptor;
Capacitation;
Acrosome reaction;
Solubilized mouse zona pellucida;
Sperm;
Mouse
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation between expression of mannose-ligand receptor(MR) on capacitated human sperm in vitro and acrosome reaction induced by zona pellucida(ZP). Methods The swimming-up sperms were incubated in capacitating medium BWW for 6 hours at 37℃, and then treated with solubilized mouse zona pellucida(mZPS).After an hour, the percentages of spermatozoa labeled with FITC-DMA were counted and used to show the expression of MR; the percentages of acrosome-reacted sperm visualized with fluoresceinated Pisum sativum agglutinin(PSA) were recorded. Results There is no correlation between expression of MR on capacitated human sperm in vitro and acrosome reaction induced by mZPS.Conclusion Expression of MR on capacitated human sperm in vitro might not be the essence of capacitation.;
