CULTURE AND PRELIMINARY STUDY OF OSTEOBLAST OF WISTAR RAT IN VITRO
- VernacularTitle:Wistar大鼠成骨细胞体外分离、培养及初步研究
- Author:
Wei TIAN
;
Shuling BAI
- Publication Type:Journal Article
- Keywords:
Bone tissue engineering;
Osteoblast;
Culture in vitro;
Biological characteristic;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To culture the osteoblast of Wistar rat in vitro and study its biological characteristics. Methods With heat-cool alternative method and by way of the trysin digestion, the osteoblast cells, obtained from femoral bone of Wistar rats, were colleced and cultured with special cultural fluid in vitro. After about two weeks, cells lined up in one layer. Then cells were generationed and examined by phase contrast microscope, H E strain, ALP strain, type Ⅰ and type Ⅲ collagen strains. Results The cells had the same morphological feature with ALP activity, type Ⅰ collagen strain positive. Conclusion The cultured osteoblasts derived from bone mass were seed cells for the bony tissue engineering, which is the base for the tissue-engineering born construction in vitro.
