SCANNING ELECTRON MICROSCOPE OBSERVATION ON CONSTRUCT OF THE COLLAGEN NETWORK IN CARBON TETRACHLORIDEINDUCED HEPATIC FIBROSIS RATS
- VernacularTitle:实验性肝纤维化大鼠胶原网络构筑的扫描电镜观察
- Author:
Ruiqiong MA
;
Shuyong ZHANG
;
Weiguang ZHANG
;
Long TIAN
- Publication Type:Journal Article
- Keywords:
Hepatic fibrosis;
Collagen fibers;
Scanning electron microscope;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore changes of three-dimensional organization of collagen fibers framework in hepatic fibrosis rats. Methods Healthy adult male Wistar rats(n=30) were randomly divided into control group(n=10) and hepatic fibrosis group.Hepatic fibrosis rats were induced by subcutaneous injections of 60% carbon tetrachloride oily solution.Rats were killed at the end of 10 week and 12 week as 10week(n=10) and 12week(n=10) hepatic fibrosis group.Distribution of collagen was detected by sirius red and polarized microscope,and the architecture was observed under the scanning electron microscope in hepatic fibrosis rats. Results Under the scanning electron microscope,with aggravation of hepatic fibrosis,kinds of collagen fibers and interlaced reticulation compartment were observed,some round or olivary !honeycomb"-like frameworks were formed,which disparted and wrapped up normal liver lobule,and the collagen fiber sheaths formed the deposition of collagen fibers surrounded portal areas or vessels.Pseudolobule vesicles formed in 12week hepatic fibrosis rats were more delicate and intact than that of in 10week hepatic fibrosis rats. Conclusion The microframework of the collagen fibers was changed in hepatic fibrosis.This structure was mainly composed of thick and slight hyperplastic collagen fibers.;
