AN EXPERIMENTAL STUDY ON THE DIRECT DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELL INDUCED BY STRIATAL ASTROCYTE
- VernacularTitle:纹状体星形胶质细胞诱导小鼠胚胎干细胞定向分化的研究
- Author:
Yuji GUO
;
Yingmao GAO
;
Lujun BING
- Publication Type:Journal Article
- Keywords:
Embryonic stem cell;
Inducing differentiation;
Dopaminergic neuron;
Striatum;
Immunocytochemistry;
Mouse
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inductive effect of striatal tissue on mouse embryonic stem cells and further analyse the cell source and inductive pattern of this inductive effect. Methods We employed striatal extracts、conditioned medium of striatal astrocytes and conditioned medium of striatal neurons to induce embryonic stem cell to differentiate directly. The differentiated cells were evaluated by morphological observation and TH immunocytochemical staining method. We also performed a quantitative analysis of the results. Results Striatal extracts and conditioned medium of striatal astrocytes had obvious inductive effect on embryonic stem cell.Percentages of three groups were 15%,15.2% and 3% respectively. Conclusions The astrocytes in striatum might have an inductive effect on dopaminergic neuronal differentiation of embryonic stem cells.The determination of inductive factor will be our next research aim.;
