EXPRESSION OF HYPOXIA INDUCIBLE FACTOR-1? AND p53   IN LUNG OF MICE DURING HYPOXIA

VernacularTitle:低氧条件下低氧诱导因子-1?及p53的表达
Author: Haibang SONG ; Longmei WA
Publication Type:Journal Article
Keywords: HIF-1?; p53; Laser confocal scanning microscope; Lung; Mice
From: Acta Anatomica Sinica 1955;0(03):-
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of hypoxia inducible factor-1? and p53 in lung during hypoxia. Methods The mice were divided into two group (n=36,male,hypoxia group=36,control group=9).The hypoxia group mice were placed in a hypoxia chamber with 10%,7%,or 5% O 2 respectively.Immunofluorescent histochemical staining technique was used.The staining results were observed under the laser confocal scanning microscope. Results HIF-1? proteins didn't express and p53 proteins expressed weakly in control group mice; Compared with the controls,the expression of HIF-1? and p53 increased dramadically.There was significant difference in expression of HIF-1? and p53 between the hypoxia groups and the control group; Positive relationship was found between the expression of HIF-1? and p53. Conclusion The expression of HIF-1? and p53 increased in lung during hypoxia,which indicates that HIF-1? may play an important role in the expression of p53 and the regulation of apoptosis.;