THE EXPERIMENTAL STUDY OF CHIMERAS CONSTRUCTION FROM EMBRYONIC STEM CELL
- VernacularTitle:小鼠胚胎干细胞构建嵌合体的方法
- Author:
Yuji GUO
;
Yingmao GAO
;
Lujun BING
;
Shengfang LI
- Publication Type:Journal Article
- Keywords:
Embryonic stem cells(ESC);
Chimera;
Microinjection;
Mouse
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To carry out construction of chimeras from embryonic stem cells of outbred KM mice. Methods We isolated embryonic stem cells from inner cell mass of KM mice blastocysts. Then we transferred embryonic stem cells into blastocoele of C57BL/6J inbred mice by microinjection in order to construct chimeric mice. Results The cells isolated from inner cell mass have typical characteristics,i e positive alkaline phophatase staining,normal karyotype,forming embryoid body. Now,we have constructed one chimeric mice successfully. Conclusion Embryonic stem cells isolated from inner cell mass can be used for the chimeras production successfully,which forms the substantial base of transgenic animal model by the way of using embryonic stem cells.
