Methylprednisolone inhibits inducible nitric oxide synthase expression in kidney of BXSB mice
- VernacularTitle:BXSB小鼠肾组织诱生型一氧化氮合酶的异常表达及甲泼尼龙干预效应
- Author:
Meimei WANG
;
Leiting XU
;
Xiaoxia ZHU
;
Rurong SUN
- Publication Type:Journal Article
- Keywords:
Lupus nephritis;
Nitric-oxide synthase;
Nitric oxide;
Methylprednisolone
- From:
Chinese Journal of Rheumatology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether inducible nitric oxide synthase(iNOS) could mediate lupus nephritis(LN) in BXSB mice and whether methyl prednisolone ameliorates LN by inhibiting iNOS expression.Methods Eighteen week old male BXSB mice (n=6) that had clinical diagnosis of glomerulonephritis were treated(i.p.) for 3 weeks with methylprednisolone(25 mg?kg-1?day-1).Controls were age- and sex-matched BXSB mice (n=6) that received normal saline only.Age- and sex- matched BALB/C mice (n=6) were used as normal controls that were treated in the same way as the BXSB controls.iNOS expression was assessed by RT-PCR and immunochemistry.Proteinuria and urinary nitrite/nitrate production were also evaluated.Results iNOS was strongly expressed in kidneys of all BXSB mice,while no signal of expression was found in kidneys of BALB/C mice by both RT-PCR and immunochemistry.Methylprednisolone reduced iNOS expression(P
