The regulating effects of interleukin-1?(IL-1?) on gene expression of Bax mRNA in human hyaline chondrocyte
- VernacularTitle:白细胞介素-1?对人软骨细胞BaxmRNA表达的作用
- Author:
Qinghua ZHAO
;
Jianhong QI
;
Qiaoling SUN
- Publication Type:Journal Article
- Keywords:
Interleukin-1;
Joints;
Chondrocyte;
Cell cultured;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Rheumatology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the regulating effects of interleukin-1? (IL-1?) on gene expression of Bax mRNA in human hyaline chondrocyte.Methods Hyaline chondrocytes of human were harvested enzymatically and cultured in DMEM supplemented with 20% bovine serum.In the experiment,various concentrations of IL-1? were added to the medium.The effects of IL-1? on the Bax mRNA were assessed by reverse transcriptase polymerase chain reaction (RT-PCR) in the passaged monolayer cell cultures of hyaline chondrocyte.Results IL-1? increased the Bax mRNA level in passaged cultures of hyaline chondrocyte.The difference was significant (P
