Influence of transfonning growth factor -?2 on production of tumor necrosis factor -? and interferon-? from placenta of intrahepatic cholestasis of pregnancy
- VernacularTitle:转化生长因子?2对妊娠肝内胆汁淤积症患者胎盘组织分泌肿瘤坏死因子?及?干扰素的影响
- Author:
Ying AI
;
Shuyun LIU
- Publication Type:Journal Article
- Keywords:
Pregnancy complications;
Cholestasis, intrahepatic;
Placenta;
Transforming growth factor-beta;
Tumor necrosis factor;
Interferon type Ⅱ
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of transforming growth factor-?2(TGF-?2) on production of tumor necrosis factor -? (TNF-?) and interferon-? (IFN-?) from placenta of patients with intrahepatic cholestasis of pregnancy (ICP). Methods Villous explants from 10 ICP patients(ICP group) and 10 normal gravidae (control group) were cultured in vitro. The concentrations of TNF-? and IFN-? in the culture medium were determined by enzyme linked immunoadsorbent assay ( ELISA) after incubation with different concentrations of TGF-?2 for 1,24 and 48 hours. Results (1) After cultured for 1,24 and 48 hours, TNF-? level in ICP group was (771 ? 187)pg/g, (2 490 ?575)pg/g,and (3339 ? 1106)pg/g, respectively, while IFN-? level was (931 ? 148) pg/g, (1888 ? 545) pg/g, and (3027 ? 667) pg/g, respectively. All were significantly higher than those in the normal group (P
