Active Immunization Study of Colon Cancer Derived 1-8D Peptide in HHD Mice.
- Author:
Hun Soon JUNG
1
;
In Sook AHN
;
Hyung Ki DO
;
Francois A LEMONNIER
;
Kuk Hyun SONG
;
Myoung Sool DO
Author Information
- Publication Type:Original Article
- Keywords: 1-8D gene; TAA (Tumor-associated antigen); Active immunization; HHD mice; Colon cancer; Peptide vaccine
- MeSH: Animals; Antigens, Tumor-Associated, Carbohydrate; Colon*; Colonic Neoplasms*; Humans; Immunization; Immunotherapy; Interferons; Mice*; Peptides; Vaccination*
- From:Immune Network 2005;5(3):157-162
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: 1-8D gene is a member of human 1-8 interferon inducible gene family and was shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from human 1-8D gene were shown to have immunogenicity against colon cancer. METHODS: To study tumor immunotherapy of three peptides we established an active immunization model using HHD mice. D(b-/-) x beta2 microglobulin (beta2 m) null mice transgenic for a chimeric HLA-A2.1/D(b-)beta2 m single chain (HHD mice) were challenged with B16/HHD/1-8D tumor cells and were immunized with irradiated peptide-loaded RMA- S/HHD/B7.1 transfectants. In therapy model tumor growth was retarded in HHD mice that were injected with 3-5 peptide-loaded RMA-S/HHD/B7.1. In survival test vaccination with 1-8D-derived peptide protects HHD mice from tumor progression after tumor challenge. RESULTS: These studies show that peptide 3-5 derived from 1-8D gene can be the most effective candidate for the vaccine of immunotherapy against colon cancer and highlight 1-8D gene as putative colon carcinoma associated antigens. CONCLUSION: We demonstrated that RMA-S/HHD/ B7.1 loaded with 1-8D peptides, especially 3-5, immunization generates potent antitumor immunity against tumor cells in HHD mice and designed active immunization as proper immunotherapeutic protocols.
