Acute Deafness Induced by Co -administration of Kanamycin Sulfate and Furosemide in a Rat Animal Model
10.3969/j.issn.1006-7299.2014.03.012
- VernacularTitle:硫酸卡那霉素联合呋塞米快速致聋大鼠模型的研究
- Author:
Hui YAN
;
Wei XING
;
Yongli SONG
;
Renfeng WANG
;
Jun CHEN
;
Jianhua QIU
- Publication Type:Journal Article
- Keywords:
Kanamycin sulfate;
Furosemide;
Rat;
Deafness;
Animal model
- From:
Journal of Audiology and Speech Pathology
2014;(3):277-280,281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of co -administration of the loop diuretic furosemide (Fur) and kanamycin (KM ) in the rats in order to establish a reliable animal model of sensorineural hearing loss .Methods Thirty -two Sprague-dawley (SD) rats were randomly divided into 4 groups with 8 rats in each group .Rats in group A ,B and C received a single intraperitoneal injection of kanamycin sulfate (KM ,500 mg/kg) ,followed by an-other intraperitoneal injection of furosemide (Fur ,0 .2 ml/kg ) 30 minutes later .While ,rats in control group D re-ceived same doses of normal saline .Auditory brain responses (ABRs) were recorded at 1 ,7 and 14 day after the in-jections ,which were group A ,group B and group C ,respectively .Hair cell loss ,the spiral ganglions and microstruc-ture were observed by immunofluorescence and scanning electron microscopy .Results The ABR thresholds in group A ,B and C were significantly higher than that of in control group D (P<0 .05) .There were no differences of ABR thresholds among group A ,B and C(P>0 .05) .Immunofluorescence and scanning electron microscopy showed obvi-ous hair cell loss in the basal turn in each kanamycin groups ,with cilia disarrangement and fusion ,compared to the same areas in animals from the control group .The expression of cleaved caspase -3 significantly increased in each experimental group than that of in the control group(P<0 .05) .Conclusion Co -administration of furosemide and kanamycin intraperitoneally induces profound hearing loss in rats and is an effective method of establishing acute hearing loss model in animal experiments .
