Experimental study on the relationship between protein kinase C isoforms ?、? and diabetic retinopathy
- VernacularTitle:蛋白激酶C同工酶?、?与糖尿病视网膜病变关系的实验研究
- Author:
Bo ZHOU
- Publication Type:Journal Article
- Keywords:
Protein kinase C;
Isoforms;
Vessel;
Diabetes Mellitus
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of protein kinase C (PKC) isoforms ?,? in diabetic rats and evaluate the relation between altered PKC ?,? expression and the pathogenesis of diabetic retinopathy (DR).Methods:Wistar rats were randomly divided into control group and diabetic group induced by streptozotocin;osmotic shock method was used to isolate the retinal vessels of rats;and PKC isoforms ?,? espression were determined after 2 weeks,2 months,4 months and 6 months follw-up.DR was assessed by quantitative morphometry of capillary bed.Results:Diabetes could induce the translocation of PKC isoform ? from cytosol to membrane pool after 2 weeks,2 months,4 months and 6 months,but ? isoform translocated from membrane to cytosol pool in retinal vessels of diabetic rats after 2 weeks,2 months,4 months and 6 months.Diabetic rats showed pericyte decrease and capillary basement thickening in deep capillary bed of retina after 6 months follow-up.Conclusion:The expression of PKC isoforms ?,? varies in different stages of DR,and the effect of PKC isoforms on the pathogenesis of DR is different.
