The expression of open reading frame of unknown KH gene from K562 cells in E.coli
- VernacularTitle:K562细胞未知基因KH的开放阅读框架在大肠杆菌中的表达
- Author:
Yujuan HE
- Publication Type:Journal Article
- Keywords:
K562 cells;
KH gene;
Open reading frame;
Prokaryotic expression
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To construct the prokaryotic expression system of open reading frame of KH gene (KH-ORF),an unknown gene in K562 cells.Methods:The 225bp KH-ORF cDNA was cleaved from plasmid pGEM-T-easy-KH-ORF with EcoRI and SalI and subcloned into the pCI-neo Mammalian Expression Vector cutted with the same restriction endonucleases.The recombinant expression plasmid was transfected into BL21 (DE3) plysS by hot-shock assay.Induced by IPTG, the recombinant protein product was tested by SDS-PAGE.Results:24kD KH-ORF product was obtained as inclusion bodies in BL21 (DE3) plysS after the expression vector had been induced by IPTG for 30 minutes Conclusion :The construction of prokaryotic expression system of KH- ORF is successful.
