Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury
- VernacularTitle:急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究
- Author:
Feng XU
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Pulmonary surfactant;
Rat
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the alteration trend of pulmonary surfactant(PS) and surfactant-associated protein(SP) in rats with LPS-induced acute lung injury(ALI).Methods:Fifty-six adult Wistar rats were randomly divided into normal saline(NS) group and ALI group.The levels of mRNA of the SP-A,SP-B were measured by reverse transcription polymerase chain reaction(RT-PCR) during iv LPS at 1,3,5,7h.The content and component of PS in the bronchoalveolar lavage fluid (BALF) were measured by high performance liquid chromatography (HPLC).In addition,studies on lung dry/wet weight ratio,the protein content of BALF,alveolar oxygen partial pressure (PaO 2)and histological changes were performed.Results:Compared with the NS group,ALI group developed severe lung damage.Edema,hemorrharge and inflammatory changes were found.Total phospholipids (TPL) of BALF at 1,3,5,7h were lower than those in the NS group( P
