Expression and significance of apoptosis-related protein Fas/FasL and BcL-2 in autoimmune thyropathy
- VernacularTitle:凋亡相关蛋白Fas/FasL和BcL-2在自身免疫性甲状腺病中的表达及意义
- Author:
Chun LIU
- Publication Type:Journal Article
- Keywords:
Autoimmune thyropathy;
Fas/FasL;
BcL-2;
Immunohistochemistry
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of apoptosis-related protein Fas/FasL and BcL-2 and their significance in Hashimoto's thyroiditis (HT) and diffuse toxic goiter(DTG).Methods:Immunohistochemical staining(S-P method) was used to detect expression of apoptosis-related protein in formalin-fixed and paraffin-embeded tissues from 30 cases of HT,25 cases of DTG,and 22 normal thyroid tissues.Results:Fas-positive thyrocytes were observed in HT and DTG,while expression of Fas protein in normal thyroid tissues was negative.The positive rates of Fas expression were 40% and 60% in HT and DTG respectively,compared with the normal thyroid tissues,there was a significant difference ( P 0.05).Expressions of BcL-2 in normal thyroid tissues and DTG were stronger,while no significant correlation was found between them.In HT the staining of follicles for BcL-2 was very weak, and there was significant difference compared with that in the normal thyroid tissues and DTG.Conclusion:LIU Chun,et al (Department of Internal Medicine,College of Clinical Medicine,Chongqing Medical University) Abstract Objective:To investigate the expression of apoptosis-related protein Fas/FasL and BcL-2 and their significance in Hashimoto's thyroiditis (HT) and diffuse toxic goiter(DTG).Methods:Immunohistochemical staining(S-P method) was used to detect expression of apoptosis-related protein in formalin-fixed and paraffin-embeded tissues from 30 cases of HT,25 cases of DTG,and 22 normal thyroid tissues.Results:Fas-positive thyrocytes were observed in HT and DTG,while expression of Fas protein in normal thyroid tissues was negative.The positive rates of Fas expression were 40% and 60% in HT and DTG respectively,compared with the normal thyroid tissues,there was a significant difference ( P 0.05).Expressions of BcL-2 in normal thyroid tissues and DTG were stronger,while no significant correlation was found between them.In HT the staining of follicles for BcL-2 was very weak, and there was significant difference compared with that in the normal thyroid tissues and DTG.Conclusion:Our results suggest that the follicular cells in HT undergo apoptosis through expression of Fas/FasL and interaction between them.The down-regulation of BcL-2 expression in thyrocytes can decrease BcL-2-mediated suppression for apoptosis.Overexpression of BcL-2 in DTG could render thyrocytes resistant to Fas/FasL-mediated apoptosis.We conclude that the expression of apoptosis-related protein plays an important role in the pathogenesis of HT and DTG.
