Effects of combination of arsenic trioxide and octreotide on proliferation and cellular apoptosis in human hepatocellular carcinoma
- VernacularTitle:三氧化二砷与奥曲肽联用抑制人肝癌细胞株的增殖和诱导凋亡的作用
- Author:
Xia ZHANG
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
Octreotide;
Anticancer drug combination;
Proliferation;
Apoptosis;
Hepatocellular carcinoma
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of combination of arsenic trioxide and octreotide on the proliferation and apoptosis of hepatocellular carcinoma cells(HCC),and to explore the mechanism of their action on liver cancer.Methods:Heptocellular carcinoma cell SMMC-7721 was treated with arsenic trioxide and octreotide.Cell survival rate was determined by MTT assay;cell cycle and the apoptosis index were determined by flow cytometry (FCM);the morphological changes were observed by transmission electron microscope.Results:The combination of arsenic trioxide and octreotide significantly inhibited the growth of SMMC-7721 and the concentration of each drug in combination is 2.022?mol/L and 8.088?mol/L that was less than single use of arsenic trioxide (3.707?mol/L)and octreotide (22.637?mol/L).The combination of two drugs can arrest HCC in S phases and induce apoptosis.Conclusion:Arsenic trioxide can enhance the antiproliferative effect of octreotide to human HCC and induce apoptosis.
