A light and an electron microscopy study on inhibition of Eleo-Zedoary to human endometrial cancer
- VernacularTitle:莪术对人子宫内膜癌作用的光镜及超微结构研究
- Author:
Hua ZHAO
- Publication Type:Journal Article
- Keywords:
Eleo-Zedoary parenteral solution;
Human endometrial cancer cell line RL-952;
Apoptosis;
Necrosis
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibition of Eleo-Zedoary solution to human endometrial cancer cell line RL-952 in vitro and its possible mechanism.Methods:Using the MTT assay to display the sensitivity to the drug,the inhibition of Eleo-Zedoary parenteral solution to RL-952 cells in vitro was observed;and using light microscope and transmission electron microscope,the morphological alterations of the cells were observed.Results:The inhibition rate of Eleo-Zedoary parenteral solution to RL-952 indicated both dose-dependent and time-dependent anti-proliferative effect.Microscopically,the large vacuoles in the cytoplasm of the cells were found.Ultramicroscopically,many fat-like drops and obvious swelling of organelles as well as the mitochondrial cristaes in the cytoplasm of cells were observed.In addition the nucleus concentration and the chromatin fragmentation and coagulation,including the apoptotic bodies were observed.Conclusion:Eleo-Zedoary parenteral solution can inhibit the proliferation of human endometrial cancer cell line RL-952 by induing apoptosis and secondary necrosis.
