The Purification and Immunogenicity of TB-14 Recombinant Protein of Mycobacterium tuberculosis.
10.4046/trd.2006.61.3.239
- Author:
Ho Yeon SONG
1
;
Young Hee KIM
;
Chang Hwan KIM
;
Young Ki MIN
;
Dae Joong KIM
;
Kwang Kjune KO
Author Information
1. Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan, Korea. songmic@sch.ac.kr
- Publication Type:Original Article
- Keywords:
Tuberculosis;
TB-14 recombinant protein;
IFN-gamma
- MeSH:
Amino Acids;
Clone Cells;
Goats;
Healthy Volunteers;
Humans;
Immunity, Cellular;
Mycobacterium tuberculosis*;
Mycobacterium*;
Protein Sorting Signals;
Tuberculosis
- From:Tuberculosis and Respiratory Diseases
2006;61(3):239-247
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Culture filtrate proteins secreted by mycobacteria are thought to play an important role in inducing protective immunity and to develop new methods for diagnosing tuberculosis. METHODS: A culture filtrate protein of M. avium that was strongly reactive with goat antiserum against M. intracellulare was constructed. Its homologous protein (TB-14) in M. tuberculosis was cloned, expressed and purified. The inductions of IFN-gamma stimulated with 10 microgram of TB-14 recombinant protein and 10 microgram PPD were estimated by using whole bloods from seven PPD (-) subjects, seven PPD (+) healthy volunteers and nine tuberculosis patients. RESULTS: M. avium culture filtrate protein was confirmed as a hypothetical protein that was termed contig 116. A novel 14-kDa recombinant protein (TB-14) of M. tuberculosis was composed of 148 amino acids, including 30 amino acids of the signal peptide, and it showed 78% homology with M. avium. In the PPD (+) healthy volunteers, recombinant TB-14 protein strongly induced the secretion of IFN-gamma in whole blood cultures. CONCLUSION: These results suggest that TB-14 recombinant protein might play an important role in inducing cell-mediated immunity against tuberculosis. Furthermore, TB-14 protein antigen and its antiserum will be available for the development of new diagnostic tools for tuberculosis.
