Serum levels of 25OHD_3 in 489 child patients measured by reversed-phase HPLC
- VernacularTitle:HPLC检测489例血清25OHD_3结果分析
- Author:
Ping QU
- Publication Type:Journal Article
- Keywords:
Child;
25OHD 3;
HPLC
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the serum levels of 25OHD 3 in children.Methods:489 children who visited the Children's Hospital of Chongqing University Medical Sciences during 1994 to 2001 were included in the analysis.Serum level of 25OHD 3 was measured for each child by reversed-phase HPLC method,which was developed in our laboratory in early 1990s.Results:Of the 489 children, boys 287(58.69%) and girls 202(41.31%).30.67(150/489) children had serum level of 25OHD 3 above the normal range in this laboratory.23.31%(114/489) children had a serum 25OHD 3 below the normal range.The majority came from the Department of Primary Child Care of this hospital.77.86%(102/131) children clinically diagnosed as hypervitamin D were proved by our 25OHD 3 measurement,while only 37.29%(44/118) children clinically diagnosed as vitamin D deficiency had their serum 25OHD 3 below the normal range.In bone fracture patients,10.34%(3/29) children were found to be at lower level of serum 25OHD 3.Conclusion:Pediatricians should realize the possibility of herpervitamin D caused by incorrect treatment of rickets.The diagnosis of rickets,i.e.vitamin D deficiency,could not be just based on its clinical manifestations.25OHD 3 measured by HPLC is important for making correct diagnosis.
