The establishment of the three-dimensional culture model of the human skin squamous-celled carcinoma and their biological evaluation of the high-invasive squamous carcinoma cells
10.3969/j.issn.1671-8348.2013.29.001
- VernacularTitle:人皮肤鳞状细胞癌体外三维模型的建立及高侵袭性鳞癌细胞的生物学评价
- Author:
Xiaokun YANG
;
Yadong YANG
;
Guihong YANG
;
Shuqian TANG
;
Jinjin WU
- Publication Type:Journal Article
- Keywords:
three dimensional culture;
tissue engineering skin;
carcinoma,squamous cell;
high-invasive
- From:
Chongqing Medicine
2013;(29):3465-3467,3471
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the three-dimensional culture model of the human skin squamous-celled carcinoma ,and isolate the highly invasive cell subsets ,and compare their biological characteristics .Methods By using the composite chitosan tissue engi-neering skin ,the three-dimensional culture model of the human skin squamous-celled carcinoma was successfully established , through digestion ,we divided and got the high-invasive squamous carcinoma cells subsets .After that ,the different invasive proper-ties of subpopulations of cells in nude mice tumor effects were compared .Results Different concentrations of cinnamaldehyde and interferon on skin squamous-celled carcinoma cell proliferation inhibition had statistical significance (P<0 .01) ,the different invasive characteristics of skin squamous cells carcinoma between subpopulations of cells proliferation inhibition also was statistically signifi-cant(P<0 .01) ,and the two different invasive properties of subpopulations of cells in the tumor characteristics also had statistical significance(P<0 .01) .Conclusion We established the three-dimensional culture model of the human skin squamous-celled carcino-ma successfully ,by using this model ,we could screen the higher invasive cells subsets .
