Determination of Emodin Content in Shengfa Granule by HPLC
- VernacularTitle:高效液相色谱法测定生发颗粒中大黄素的含量
- Author:
Ping JIANG
;
Wenqing WANG
;
Chen WANG
- Publication Type:Journal Article
- Keywords:
Shengfa Granule;
Emodin;
HPLC;
Determinaton
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of emodin content in Shengfa granule.Methods HPLC was used.Mobile phase consisted of methanol-0.25 %phosphoric acid (80 ∶20) with UV detection at 254 nm,the analytical column was YMC-Pack ODS-A.Results The linear range of emodin was 0.021 ?g～0.155 ?g(r=0.9999)while the average recovery rate was 99.03 %(RSD=1.40 %,n=6).Conclusion The methods is suitable for determination of emodin content in Shengfa granule.
