Effects of hyperbaric oxygenation on the activity of antioxidation enzymes and the permeability of blood-brain barrier in mice with cerebral ischemia-reperfusion
- VernacularTitle:高压氧对脑缺血再灌注小鼠抗氧化酶类及血脑屏障通透性的影响
- Author:
Hong ZHAO
;
Xiaomei LU
;
Xuexin CHEN
;
Haipeng ZHANG
- Publication Type:Journal Article
- Keywords:
Antioxidation enzymes;
Cerebral ischemia-reperfusion;
Astrocyte;
Hyperbaric oxygenation;
Blood brain barrier
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of hyperbaric oxygenation (HBO) on generation of free radicals and the permeability of blood-brain barrier(BBB) during cerebral ischemia-reperfusion(CIR). Methods Three hundred and twenty Kunming mice were randomly divided into four groups: a sham operation group, a HBO group, a CIR group, and a HBO + CIR group, with 80 mice in each group. Conscious mice were used to establish the CIR model, and 0.25MPa (ATA) HBO were applied 5 times after operations. The activities of SOD, CAT, GSH-PX and the concentration of MDA, EB in the cerebral hippocampal tissues in each group were measured with colorimetry. The cerebral hippocampal tissues were harvested and processed, then observed and compared with transmission electron microscopic observation. Results Compared with the sham operation group, the activities of antioxidation enzymes (GSH-PX,SOD, CAT) in CIR group decreased significantly (P
