High-performance capillary electrophoresis method for ferulic acid assay in serum of rats treated with Buyang Huanwu Decoction
- VernacularTitle:喂饲补阳还五汤的大鼠血清中阿魏酸的高效毛细管电泳分析
- Author:
Qizhi LUO
;
Li TONG
;
Mengmeng YANG
;
Xuefeng XING
;
Ande MA
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction (BHD);
ferulic acid;
high-performance capillary electrophoresis (HPCE)
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a high-performance capillary electrophoresis method to determine ferulic acid concentrations in serum of rats treated with Buyang Huanwu Decoction (BHD). Methods Capillary zone electrophoresis was applied for ferulic acid assay, quantitative determination was based on internal standard and detection was carried on by direct UV. The electrolyte buffer was composed of 25 mmol/L borax-methanol (85∶15). Capillary electrophoresis was performed using a 52 cm (30 cm to detector)?50 ?m fused-silica capillary tube. Separation voltage was 20 kV, sampling time was 3 s, detection wavelength was 320 nm, and the temperature was 30 ℃. Results Ferulic acid was successfully separated within 6 min, the recoveries were 97.2% in serum and 103.28% in BHD, respectively. RSD were 3.73% and 0.91% (n=3), respectively. Conclusion This method can supply reference for the determination of ferulic acid in serum samples and BHD.
