Clinical study on perioperative change of myocardial NF-?B nuclear translocation in cardiopulmonary bypass
- VernacularTitle:围体外循环期心肌细胞核因子?B活性变化的临床研究
- Author:
Jianjun QIAN
;
Hua JING
;
Demin LI
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary bypass NF-kappa B TNF-? Myocardial inflammatory activation
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To seek the direct evidence of myocardial inflammatory activation in a cohort of patient undergoing surgery on cardiopulmonary bypass (CPB). Methods: Twenty patients undergoing surgery on CPB had plasma samples obtained for tumor necrosis factor-? (TNF-?), perioperatively. Myocardial tissue samples were obtained intraoperatively, both pre-and post-CPB. Myocardial nuclear factor ?B (NF-?B) nuclear translocation and plasma TNF-? concentrations were analyzed by standard enzyme-linked immunosorbent assay (ELISA). Cross clamp times, CPB times and the time to extubation were recorded. The change of myocardial NF-?B nuclear translocation and plasma TNF-? concentrations was assessed by analysis of variance (ANOVA). The regression analysis was utilized to define the correlations among the optical densities of NF-?B, concentrations of plasma TNF-?, influencing factors and heart function. Results: (1) Seventeen patients had NF-?B nuclear translocation before initiation of CPB, the optical density (OD) of myocardial NF-?B was significantly increased at the end of CPB in nineteen patients. (2) The correlation between the activity of NF-?B and concentration of TNF-? was signi ficant. (3) The correlation between the activity of NF-?B and clinic outcomes and other influencing factors was not significant. Conclusion: Myocardial inflammatory activation may be present in the patients before CPB. CPB could increase the activity of myocardial NF-?B.
