EFFECTS OF INTERLEUKIN-1? ON EXPRESSION OF GROUP Ⅱ mGluR2/3 mRNA IN THE HIPPOCAMPUS OF EPILEPTIC RATS INDUCED BY GLUTAMATE
- VernacularTitle:白细胞介素1?对谷氨酸致痫大鼠海马mGluR2和mGluR3 mRNA表达的影响
- Author:
Wenping GUO
;
Changgeng ZHU
;
Qingying LIU
- Publication Type:Journal Article
- Keywords:
Interleukin-1?;
RT-PCR;
Metabotropic glutamate receptors;
Hippocampus;
Epilepsy;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between interleukin-1?(IL-1?) and group Ⅱ metabotropic glutamate receptors(mGluR2/3). Methods The rats were randomly divided into five groups:1^cotrol;2^group Glu;3^group IL-1?+Glu;4^group IL-1?;5^group IL-1?+D-AP5+Glu.The behavior of animals was observed.Expression of mGluR2/3 mRNA was assayed with RT-PCR. Results The alteration of behavior showed that the latent period of seizure in group with IL-1?+Glu was shortened significantly and the degree of seizure was also more serious as compared with that in group Glu.Expression of mGluR2/3 mRNA showed marked enhancement in group with IL-1?+Glu and group with Glu compared with control.However,expression of mGluR2 mRNA in group IL-1?+Glu was markedly decreased as compared to that in group Glu;the level of mGluR3 mRNA showed no apparent difference between group IL-1?+Glu and group Glu.Conclusion Present study suggests that IL-1? enhances the degree of seizure induced by glutamate,the mechanism may be due to its direct or indirect effects on down-regulation of expression of mGluR2 mRNA.
