THE EFFECT OF THE INTEGRITY OF NEUROFILAMENT ON THE SHAPE AND DISTRIBUTION OF NEMATOLYSOSOME
- VernacularTitle:神经丝的完整性对线状溶酶体形状与分布的影响
- Author:
Ying HUANG
;
Da LIU
;
Yuxi SHI
- Publication Type:Journal Article
- Keywords:
Neurofilament;
Nematolysosome(NLY);
Vanadate;
Neuron culture;
Immunocytochemistry;
Electon microscopy;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm that if the integrality of NF has effect on the shape and distribution of NLY. Methods using SABC monocolon antibody immunohistochemistry,electron microscopy,immunocytochemistry, and enzymocytochemistry to observe the disorder of NF-H and the changes of distribution and shape of NLY after vanadate treated. Results When the integrality of NF was damaged, the proteins of NF gathered towards nuclear accompany with the similar movements of NLY, meanwhile the shape of NLY also changed into round from thread-like shape.Conclusion Through different ways we used, vanadate can over-phosphate NF proteins and destroy the assemble ability. Whether NF has a complete structure is important to the shape and distribution of NLY, which will be changed when the structure of NF is disorganized.
