THE BIOLOGICAL FEATURES OF RHESUS BONE MARROW MESENCHYMAL STEM CELLS
- VernacularTitle:恒河猴骨髓间充质干细胞的生物学特性
- Author:
Qiwei WANG
;
Wanshan WANG
;
Yingjie PIAO
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Flow cytometry;
In situ hybridization;
Immunocytochemistry;
Rhesus
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to explore the biological features of bone marrow mesenchymal stem cells. Methods The ALPase activity, Vimentin and Laminin were detected by immunocytochemistry. Cell cycle and surface antigenic features were analyzed by flow cytometry technique. Cell growth curve was measured by MTT test. The telomerase activity was detected by in situ hybridization. Results The ALPase activity and Laminin were negative, Vimentin was positive in the MSCs. The percentage of G1, S, and G2 of cell cycle was 89.5%,7.8%,7.7%, respectively. MSCs were positive for CD166、CD29、CD44、CD105, and negative for CD34 and HLA-DR. The average doubling time of cells was 32 hours. The telomerase activity was positive. Conclusion The cultured cells were not hemopoietic stem cells or fibroblasts, but the MSCs that had not differentiated. Furthermore, it had relatively high proliferation ability.
