AN EXPERIMENTAL STUDY ON THE THERAPY OF PARKINSON DISEASE BY NEUROEPITHELIAL CELL TRANSPLANTATION INTO RAT BRAIN
- VernacularTitle:大鼠胚胎神经上皮细胞脑内移植治疗帕金森病的实验研究
- Author:
Jinhao SUN
;
Yingmao GAO
;
Lin YANG
;
Zhenhua LI
- Publication Type:Journal Article
- Keywords:
Neuroepithelial cell;
Parkinson disease;
Brain transplantation;
Embryo;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Embryonic neuroepithelial cells (NEC) were transplanted into the brains of Parkinson disease rats, the survival and differentiation of NEC were investigated. Methods Embryonic rats (E11) were obtained from the pregnant Wistar rats. Neuroepithelial cells were dissociated from embryonic neural tube and treated with 0^25% trypsin, then transplanted them into the striatum of Parkinson disease (PD) rats stereotaxicly. Coronal sections of the brain were made in cryostat. The survival of transplant neuroepithelial cells and tyrosine hydroxylase (TH) expression of some NEP were examined by immunocytochemical technique, also the rotation behavior was tested following different time of transplantation. Results Some grafts were found survived well and some TH-positive cells could be detected in the core of graft region. NEP transplantation could also partly improve the symptoms of PD rats.Conclusion The results demonstrate that embryonic stem cells dissociated from neural tube can survive and differentiate into dopaminergic neurons. NECs contribute to the behavioral recovery.
