AN EXPERIMENTAL STUDY ON ALCOHOL-INDUCED GOLDEN HAMSTER NEURAL TUBE DEFECTS AND EYE ABNORMALITIES
- VernacularTitle:酒精致金黄地鼠神经管和眼畸形的实验研究
- Author:
Ling LI
;
Wenqing WANG
- Publication Type:Journal Article
- Keywords:
Teratogenesis;
Alcohol (ethanal);
Neural tube defects;
Eye abnormalities;
Apoptosis;
Golden hamster
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the morphological changes in alcohol-induced pregnanted golden hamster neural tube defects and eye abnormalities, and to study quantitatively the alterations of apoptosis during teratogenesis. Methods The pregnanted golden hamsters were killed 4,8,16,24, 48 and 72 hours after teratogenic dose alcohol exposure respectively, and both embryos and fetuses were obtained. The LM, SEM and stereomicroscope were used to observe the histological alterations during the teratogenesis of the neural tube and the eye. With the TUNEL and TEM methods, the apoptosis were studied qualitatively. Results 1^The closure of the golden hamster neural tube and the formation of the optic cup were significantly retardation in the experimental groups. 2^After 8h of maternal alcohol exposure, the apoptosis of neural epithelial cells increased apparently, especially in the prosencephalon and neural crest.At either 16h or 24h after treatment, the index of apoptosis was also higher than that in the control groups, and the apoptosis chiefly located in the neural crest and optic grooves.After 48h, this index gradually declined to the level of control groups.Conclusion Alcohol can delay the closure of the golden hamster neural tube and the formation of the optic cup. Meanwhile it can cause excessive apoptosis on these structures.These alterations are probably partially involved in the mechanism of alcohol-induced neural tube defects and eye abnormalities.
