The expression and activation level of nuclear factor?B in synovium of rheumatoid arthritis
- VernacularTitle:核因子?B在类风湿关节炎滑膜组织中的表达与意义
- Author:
Fei HAN
;
Xin YOU
;
Fulin TANG
- Publication Type:Journal Article
- Keywords:
Arthritis rheumatoid;
Nuclear factor ?B;
Interleukin-1?;
Matrix metalloproteinase 9
- From:
Chinese Journal of Rheumatology
2002;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression and activation level of nuclear factor ?B (NF-?B) in synovium of rheumatoid arthritis (RA).Method Forty-six specimens including 17 RA, 24 osteoarthritis (OA) and 5 normal synovial tissues were subjected to RT-PCR to determine the mRNA level of NF-?B p65,p50,inhibitor of NF-?B (I?B),interleukin-1? (IL-1?) and matrix metalloproteinase 9 (MMP-9).Thirty-nine sections including 14 RA,21 OA and 4 normal synovium were subjected to immunohistochemistry to determine the expression level of NF-?B p65.Synoviocytes from 14 synovial specimens including 8 RA and 6 OA were cultured and the nuclear protein was extracted and reserved for western blot.Results The mRNA level of p65,IL-1(,MMP-9 were higher in RA group than that of control (P
