Change of expression of angiotensin Ⅱ type 1 receptor in the formation of liver fibrosis
- VernacularTitle:血管紧张素Ⅱ1型受体在肝纤维化形成过程中表达变化的研究
- Author:
Weiwei WANG
;
Xishan YANG
;
Xu LI
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ type 1 receptor;
Liver fibrosis;
Hepatic stellate cell;
Expression of gene;
Reverse transcription polymerase chain reaction
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of angiotensin Ⅱ type 1 receptor (AT1R) in the different fibrotic stages of human liver. Methods The collagen Ⅰ was detected by immunohistochemical method. Indirect immunofluoresence-labelled method was used to detected AT1R. And semi-quantitative RT-PCR was used to detect AT1R mRNA. Results The type Ⅰ collagen area enlarged with the progression of hepatic fibrosis. The positive expression of AT1R was concentrated in the cytoplasm of hepatic stellate cells(HSC) around the lobes of liver or in the hepatic sinus. Eight of twelve cases of normal liver tissues were positive, and all the eighteen cases of fibrotic liver tissues were positive. The positive cell number in fibrotic liver tissues was significantly higher than that in normal ones (P
