Detection of hMLH1/hMSH2 expression for identifying patients with hereditary nonpolyposis colorectal carcinoma
- VernacularTitle:hMLH1和hMSH2蛋白表达在遗传性非息肉病性大肠癌诊断中的应用
- Author:
Sanjun CAI
;
Ye XU
;
Hongfen LU
- Publication Type:Journal Article
- Keywords:
Immunohistochemistry;
Hereditary honpolyposis colorectal carcinoma;
hMLH1;
hMSH2
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of expression of hMLH1 and hMSH2 in detecting hereditary nonpolyposis colorectal carcinoma (HNPCC). Methods Colorectal carcinomas from 66 patients were examined by immunohistochemistry for the expressions of hMLH1 and hMSH2 using commercially available monoclonal antibodies. Immunohistochemical patterns of tumors from 19 HNPCC patients (Group A), 20 suspected HNPCC patients (Group B), 14 patients whose clinical features conformed to the Bethesda guideline (Group C), and 13 sporadic colorectal cancer patients (Group D) were compared. Results The absence or low expression of hMLH1 and hMSH2 was revealed in 72.8% patients of group A, 60.0% in group B, 28.4% in group C and 7.7% in group D. The absence or low protein expression of hMLH1 and hMSH2 was significantly correlated with HNPCC (P=0.0008). The absence or low expression of hMLH1 was higher detection rate than that hMSH2 (P=0.01). Conclusions HNPCC patients can be identified by immunohistochemical methods according to the expression of hMLH1 and hMSH2, which may be used in clinical practice and research. Immunohistochemical analysis of hMLH1 and hMSH2 may predict the presence of corresponding gene mutations. Gene mutation in hMLH1 might be higher than that in hMSH2 in Chinese HNPCC.
