Role of extracellular signal-regulated kinase in apoptosis of hepatic stellate cells induced by Arg-Gly-Asp-Ser tetrapeptide
- VernacularTitle:细胞外信号调节激酶在精-甘-天冬-丝氨酸四肽诱导肝星状细胞凋亡中的作用
- Author:
Xiaolan ZHANG
;
Huiqing JIANG
;
Xinqing LU
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cell;
Extracellular signal-regulated kinase;
Apoptosis;
Arg-Gly-Asp-Ser tetrapeptide
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of extracellular signal-regulated kinase(ERK)in the apoptosis of hepatic stellate cells(HSC) induced by Arg-Gly-Asp-Ser (RGDS) tetrapeptide. Methods The cultrued HSC cells were divided into six groups including untreated control, fibronertin (FN), FN+RGDS (25 mg/L), FN+RGDS (50 mg/L), FN+RGDS(100 mg/L) and FN+Arg-Gly-Glu-Ser(RGES, 100 mg/L) groups. 3H-thymidine incorporation, transmission electron microscopy and TUNEL were employed to estimate the influence of RGDS on proliferation and apoptosis of HSC. The HSC adhesion rates were observed by toluidine blue colorimetric assay. The expressions of ERK mRNA and ERK protein in HSC were detected by RT-PCR and Western blotting. Results Compared with control and fibronectin groups, RGDS tetrapeptide at concentrations of 25 mg/L, 50 mg/L and 100 mg/L inhibited the proliferation of HSC(P
