Determination of dihydromyricetin in different parts of Ampelopsis grossedentata in different seasons by HPLC
- VernacularTitle:HPLC测定藤茶不同采收时期及不同部位的二氢杨酶素含量
- Author:
Guixia HE
;
Gang PEI
;
Weili YANG
;
Bi LI
- Publication Type:Journal Article
- Keywords:
Ampelopsis grossedentata;
dihydromyricetin;
HPLC;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the determination of dihydromyricetin in stem,leaf of Ampelopsis grossedentata in different seasons from different habitat. METHODS : Novapak C_ 18 column (150mm?4.6mm,5?m) was selected as separation column at 25 ?C . Methanol-water-phosphoric acid (27∶73∶0.1) was used as a mobile phase at a flow rate of 1.0mL?min -1 . The peak of dihydromyricetin was detected at UV 290nm. RESULTS : The linearity of this method was good. The average recovery was 99.47%,RSD was 1.68%. The content of dihydromyricetin in leaf of Ampelopsis grossedentata collected in May was the highest and was three ～ four times the size of that in stem. CONCLUSIONS :The method is convient with a good separating degree and is useful basis for the develoment and utilization of Ampelopsis grossedentata.
