Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Jieduwanling Pills by HPLC
- VernacularTitle:HPLC测定解毒万灵丸中盐酸麻黄碱和盐酸伪麻黄碱的含量
- Author:
Yong CHEN
- Publication Type:Journal Article
- Keywords:
ephedrine hydrochloride;
pseudoephedrine hydrochloride;
Jieduwanling Pills;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method for determining ephedrine hydrochloride and pseudoephedrine hydrochloride in Jieduwanling Pills (Rhizoma atractylodis,Herba ephedrae,Herba dendrobii,etc.)by RP-HPLC. METHODS : The Agilent Extend C_ 18 column with acetonitrile-0.1%H_3PO_4(5∶95)as the mobile phase was used. The flow rate was at 1.0 mL?min -1 and the detective wavelength was at 206 nm. RESULTS : The calibration curve was linear at a range of 0.02028 ～1.014?g for the ephedrine hydrochloride( r =0.9999). the average recovery was 97.69% and RSD was 0.9%( n =6). The calibration curve was linear at a range of 0.02254 ～ 1.127 ?g for the pseudoephedrine hydrochloride( r =0.9998). The average recovery was 98.40% and RSD was 2.3%( n =6). CONCLUSION : The method is accurate with a good reproducibility and can be used as a quantitative analysis for this preparation.
