OPG/RANKL gene expression of bone marrow stromal cell under stimulation of PTHrP
- VernacularTitle:甲状旁腺素相关肽对骨髓基质细胞中RANKL及OPG基因的表达影响
- Author:
Huiren TAO
;
Quanping WANG
;
Huan LI
- Publication Type:Journal Article
- Keywords:
Osteoclasts;
Bone marrow cells;
PTHrP
- From:
Chinese Journal of Rheumatology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the OPG/RANKL gene expression of bone marrow stromal cell under stimulation of PTHrP.Method Primary bone marrow cells were cultured under the stimulation of 45 ng/ml PTHrP. Number of osteoclasts was counted after 6-day's culture.90 ng/ml PTHrP was used to stimulate adherent bone marrow stromal cells for 3 or 6 days.OPG and RANKL gene expression level were determined by real-time PCR. Result Large number of osteoclasts were formed in primary bone marrow cell culture after 6-day stimulation of PTHrP.RANKL gene expression level was up-regulated by PTHrP,while OPG gene expression level were down-regulated.Conclusion PTHrP stimulates osteoclast formation via up-regulating RANKL level and down-regulating OPG level.
