Effect of Xinmailing Injection on Lipopolysaccharide-induced Secretion of Endothelin-1 and Nitric Oxide from Endothelial Cells of Human Umbilical Veins
- VernacularTitle:心脉灵注射液对内毒素刺激人脐静脉内皮细胞ET-1和NO分泌的影响
- Author:
Shanshan LI
;
Peng LI
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Xinmailing Injection;
Endothelial cell;
Nitric oxide;
Endothelin;
Lipopolysaccharide
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Xinmailing Injection (XI) on lipopolysaccharide(LPS)- induced secretion of endothelin- 1 (ET- 1) and nitric oxide (NO) from endothelial cells of human umbilical veins.Methods The in- vitro culture of human umbilical vein endothelial cells were stimulated by LPS( 100 ? g/L) and incubated with XI(320 g /L) for half an hour,one hour,2 hours,4 and 6 hours respectively.Then the cultured supernatants were collected.The viabilities of cells were measured by MTT method.The concentrations of ET- 1 and NO were determined by radioimmunoassay method and by Gneiss's Method respectively.Results (1) The viabilities of cells arrived the highest level as treated with XI with the concentration of 320 g /L (P
