Protective effect of Prescription for Nourishing Liver- Kidney on Injured Cultured PC12 Cell in Vitro
- VernacularTitle:培补肝肾复方中药对PC12细胞损伤模型的保护作用
- Author:
Jiyu TIAN
;
Jianzong CHEN
;
Xiaoli CHEN
- Publication Type:Journal Article
- Keywords:
Prescription for nourishing liver- kidney;
PC12 cell injury model;
Sero- pharmacology;
Protective effect
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of prescription for nourishing liver- kidney (PNLK) on PC12 cell injyred by 1- methyl- 4- phenylpyridinium (MPP+ ).Methods The rabbit serum contained PNLK was preparing by the method of sero- pharmacology.PC12 cell was cloned from pheochromocytoma of adrenal medulla and the MPP+ injured models were induced by nerve growth factor.The effect of serum containing PNLK on PC12 cell from MPP+ injured models was assayed by morphological, MTT staining and LDH measurement.Results The equivalent dosage of the serum of PNLK possessed obvious protective effects on PC12 cell,increased the number of survival cells and relieved the injury significantly.Conclusion Serum of PNLK can counteract the injuryof PC12 cell induced by MPP+ and has protective effect on PC12 cell in vitro.
