Determination of Total Alkaloids in Semen Strychni and Optimization of Its Extraction Conditions by Orthogonal Design
- VernacularTitle:马钱子总生物碱的测定及其提取条件的正交设计优选
- Author:
Yanchao LI
;
Baochang CAI
;
Weidong LI
;
Liuqing DI
- Publication Type:Journal Article
- Keywords:
Semen strychni;
Total alkoloids;
Determination;
Orthogonal test;
Pharmaceutical technology,TCD;
RP- HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the RP- HPLC method for the determination of total alkaloids in Semen Strychni and to optimize its extraction conditions by orthogonal design. Methods With strychnine and brucine content as the indexes, ethonal volume, ethonal concentration , pH and extracting time were screened and L9(34) orthogonal test were applied. Results The optimal extraction conditions were as follows: refluxing and extracting with 6- fold 50 % alcohol (pH=5) for 3 times, one hour for each time. Conclusion The optimized condition is the best for the extraction of alkaloids and this increase the availability of Semen Strychni.
