Determination of Naringin in Bogu Pill by HPLC
- VernacularTitle:HPLC法测定驳骨丸中柚皮苷的含量
- Author:
Yanling LI
;
Qixun TANG
- Publication Type:Journal Article
- Keywords:
Bogu Pill;
Naringin;
HPLC;
Determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the content determination of naringin in Bogu Pill by HPLC. Methods At room temperature and by using ultrasonic extraction, HPLC was performed to determine naringin content on ODS chromatographic column.The mobile phase consisted of a mixture of acetonitrile and 8 % acetic acid (15 ∶ 85)and detection wavelength was at 283 nm.Results The linearity of naringin was in the range of 5.2 ? g / mL~ 31.2 ? g / mL (r=0.9999) and the average recovery was 98.72 % , RSD=1.55 % . Conclusion This method was simple, sensitive and accurate, and can be used for the quality control of Bogu Pill.