Determination of Chlorogenic Acid in Fuyanqing Perfusate by HPLC
- VernacularTitle:HPLC法测定附炎清灌注液中绿原酸的含量
- Author:
Xixiang LI
;
Jingyun DU
;
Lingyan WU
- Publication Type:Journal Article
- Keywords:
Fuyanqing perfusate;
Chlorogenic acid;
HPLC;
Determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of chlorogenic acid in Fuyanqing perfusate (FP). Methods The sample was extracted with methyl and the chromatographic condition were as follows: C18 chromatographic column (250 mm? 4.6 mm,5 ? m),a mobile phase of methyl alcohol and water and methyl acid(30 ∶ 70 ∶ 1),the detection wavelength at 327 nm and the flow rate being 0.5 mL/min.Results A linearity of chlorogenic acid in FP was obtained in the range of 0.16 ? g～ 0.58 ? g,r=0.9996(n=6).The average recovery was 98 % and RSD =1.0% (n=6).Conclusion This method is easy,sensitive and accurate for the determination of chlorogenic acid in FP.
