Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR)
- VernacularTitle:缺口连接酶链反应检测沙眼衣原体DNA的实验研究
- Author:
Hong WEI
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Ligase chain reaction;
Polymerase chain reaction
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction(G-LCR).Methods:A G-LCR DNA amplification assay that targeted the outer major membrane protein gene(omp1)of CT was established to detect CT infection.The sensitivity and specificity of a newly developed G-LCR test was examined by the use of highly purified elementary bodies (EBs).DNA fragments of different species and from other bacteria1 were detected with G-LCR and routine polymerase chain reaction (PCR).Results:Using G-LCR,DNA fragments of 54bp were amplified from five different species.The sensitivity could be improved to detect out 2 chlamydial elementary bodies.G-LCR detected ten-fold EBs than PCR.No signal was observed when C.pneumoniae and other bacteria were used as templates.Conclusion:G-LCR is sensitive,rapid and specific for detection of Chlamydia trachomatis.
