Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus
- VernacularTitle:单核苷酸多态性在研究糖尿病微血管病变易感基因中的应用
- Author:
Yuhong ZHANG
- Publication Type:Journal Article
- Keywords:
Type 2 diabetes mellitus;
Diabetic microangiopathy;
Aldose reductase gene;
Single nucleotide polymorphism
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the distribution of the (C-106T) single nucleotide polymorphism and genotype of promoter region of aldose reductase gene and to study its relation to the susceptibility of microangiopathy in type 2 diabetes mellitus in Chinese Han from Chongqing.Methods:The aldose reductase gene in promoter region was amplified by polymerase chain reaction and the frequency of genotype of type 2 diabetes were analyzed by restriction enzyme BfaI.Results:The frequencies of genotype CC were significantly higher and the frequencies of genotype TT were much lower in type 2 diabetes patients with microangiopathy than in those without microangiopathy (? 2=6.78 and 4.45,respectively,P
