Apoptosis of posterior capsular lens epithelial cell induced by adriamycin and the expression of apoptosis-related gene in rabbit
- VernacularTitle:阿霉素诱导兔眼后囊晶状体上皮细胞凋亡及其凋亡基因的表达
- Author:
Li LI
- Publication Type:Journal Article
- Keywords:
Adrimagcine;
Lens epithelial cell;
Apoptosis;
Protein expression;
P53;
Bcl-2
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of adriamycin(ADR) as therapeutic agent inducing rabbit posterior capsular lens epithelial cell apoptosis and to assess the relationships between P53,Bcl-2 after lens extracapsular extraction.Methods:Twelve rabbit eyes were injected with 0.2mg ADR(0.4mg?L -1 ) during lens extracapsular extraction performed respectively in the third week and the eighth week after the operation.With TdT-mediated dUTP nick end labeling(TUNEL)and immunohistochemical(SABC) staining.The protein expression cell rate of P53 and Bcl-2 were detected.Results:Positive pericyte muclei labeled by TUNEL increased more significantly than those in the control guoup from the third week to the eighth week after the operation.P53 protein expression was significantly higher(P
