In vitro experience with tissue-engineered heart valve leaflets
- VernacularTitle:组织工程心脏瓣叶体外生成可行性研究
- Author:
Jingfang ZHANG
;
Ping HU
;
Xuhua JIAN
- Publication Type:Journal Article
- Keywords:
Heart valve prosthesis Cell culture Scanning electron microscopy Histology hydroxyproline Tissue engineered
- From:Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The experiment was designed to evaluate the in vitro creation of tissue engineered heart valve leaflets. Methods: Aortic artery was harvested from 6 hybrid young pigs. The endothelial and myofibroblast cells were isolated and cultured to get enough cells for seeding. The myofibroblast and endothelial cells were seeded on the scaffolds sequentially. The cellular scaffolds were cultured 28 days and sent for scanning electron microscopy (SEM), histological and hydroxyproline examination. Results: SEM showed that a large amount of cells attached on the scaffolds. The cell had covered a large area of scaffold with a lot of matrix synthesized. The HE stain showed large amount of cells growing on the scaffolds and some cells were found in the central part of scaffolds. Gomori stain showed that there was elastin synthesized on the scaffolds. Hydroxyproline content is (10.93?2.89) ?g/g (scaffolds). Conclusion: With vascular cell, it is possible to seed them onto biodegradable scaffolds and create a viable tissue-engineered heart valve leaflets in vitro.
