NERVE GROWTH FACTOR(NGF) INDUCES DIRECTIONALMIGRATION OF NEURAL STEM CELLS
- VernacularTitle:神经生长因子诱导神经干细胞定向迁移
- Author:
Yingtang SHI
;
Ji MA
;
Chonggang YUAN
- Publication Type:Journal Article
- Keywords:
Directional migration of neural stem cell;
Semi-solid cell culture;
Nerve growth factor(NGF);
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nerve growth factor(NGF) on inducing directional migration of neural stem cells in vitro. Methods Semi-solid cell culture method was used to observe the cell′s directional migration;immunocytochemical method was used to analyse the neuronal phenotype. Results In the semi-solid medium with gradient concentration of NGF,neural stem cells migrated directionally from lower concentration of NGF to higher concentration,and the nearer to the source of NGF,the more evident the result.The migrated cells show neuron charcteristics.In the medium with NGF but with no gradient concentration,the cells migrated without direction.Conclusion NGF could induce directional migration of neural stem cells.
