RESEARCH ON THE COMMITTED DIFFERENTIATION OF EMBRYONIC STEM CELLS INTO EPIDERMAL-LIKE STEM CELLS INDUCED in vitro
- VernacularTitle:体外定向诱导胚胎干细胞分化为表皮样干细胞的研究
- Author:
Renli ZHANG
;
Shujun CHENG
;
Haibiao LI
- Publication Type:Journal Article
- Keywords:
Embryonic stem cell;
Epidermal stem cell;
Differentiation;
Amnion;
Immunohistochemistry;
Mouse
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the condition which can induce mouse ES cells to differentiate into epidermal-like stem cells for the clinical application of ES cells-derived epidermal-like stem cells and the research on the mechanism of committed differentiation of ES cells. Methods Coculture mouse ES cells with human amnion for 3-4 days, and the committed differentiation were detected by flow cytometry and immunohistochemistry. In experimental group 1, amnion was pasted covering the whole bottom of the wells, with the epithelial surface upward, and in group 2 covering the half bottom. No amnion was used in control. Results After 3-4 days of co-culture, epidermal-like stem cell clones were formed on the epithelial surface of amnion in group 1 and group 2, and expressed high levels of integrin-? 1, CK19 and CK15. The percentages of integrin-? 1, CK19 and CK15 positive cells counted in group 1 by flow cytometry were 89.2%, 86.8% and 71.2% respectively, versus the control group of 8.4%,9.6% and 11.8%, the differences were significant in all the three indices (Z tests P
