AORTIC SMOOTH MUSCLE CELLS PRODUCED ELASTIC FIBERS IN THREE-DIMENSIONAL CULTURE
- VernacularTitle:主动脉平滑肌细胞在三维培养中产生弹性纤维的研究
- Author:
Lingxiang YU
;
Zhongzhi ZOU
- Publication Type:Journal Article
- Keywords:
Aortic smooth muscle cell;
Dermis substitute;
Three-dimensional culture;
Elastic fiber;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Study if aortic smooth muscle cells(ASMC) can produce elastic fibers in three-dimensional culture imitating the dermis substitute. Methods Primary culture of ASMCs were obtained by explanting of thoracic aortae from one-week old SD rats. ?-actin immunocytochemistry was used to approve the smooth muscle cells. After subculture, ASMCs were mixed with gels composed of collagen, chitin and glucosaminoglycans, then, three-dimensional culture was carried out. After 1 week and 2 weeks, Gomori aldehyde fuchsin staining and elastin immunocytochemistry were applied to detect the elastic fibers in the ASMC-collagen gels. Results More than 98% of the cultured cells were approved as smooth muscle cells by ?-actin immunocytochemistry. After both 1 and 2 weeks of three-dimentional culture, elastic fibers were detected in the ASMC-collagen gels by both stainings.Conclusion ASMCs can synthesize elastin and produce elastic fiber in three-dimentional culture imitating the dermis substitute.
